Reason for non-specific bands from Northern?

Mengjuan Guo mguo at students.uiuc.edu
Wed Jan 30 16:14:10 EST 2002


I just did Northern on an Arabidopsis gene with Ultrahyb buffer from
Ambion, but got mutiple bands after 15 hours hybridization.  I think my
probe was specific, because when BLAST the nucleotide sepquence of this
probe, no homology was found in Arabidopsis genome. So what could be the
problem? Could the Ultrahyb be too sensitive? Thanks for help!




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