G418 selection in HEK293 cells.

delios97 at hotmail.com delios97 at hotmail.com
Wed Jan 30 20:51:03 EST 2002


Use 1 mg/ml, that will ensure killing of cells that have not
integrated the neo resistance marker 


On 31 Jan 2002 01:38:31 -0000, Zhonglin.Chai at baker.edu.au ("Zhonglin
Chai") wrote:

>Hello There,
>
>I wonder what concentration of G418 is optimal or used to select
>transfected HEK293 cells? I have not had a chance to test various
>concentrations, but 400 ug/mL (working fine with HeLa cells) did not
>seem to kill my cells after a week. Your personal experience and
>suggestions will be mostly appreciated.
>
>The Best Regards.
>
>
>
>
>
>Zhonglin Chai, PhD
>
>Molecular Hypertension Laboratory
>Baker Medical Research Institute
>P.O. Box 6492
>St.Kilda Road 
>Central Melbourne 
>Vic 8008, Australia
>Telephone: +61 3, 8532 1231
>mobile : 0413 58 1940, or international +61, 413 58 1940
>Fax: +61 3, 8532 1100 
>email: zhonglin.chai at baker.edu.au
>
>
>
>**********************************************************************
>This email and any files transmitted with it are confidential and
>intended solely for the use of the individual or entity to whom they
>are addressed. If you have received this email in error please notify
>the system manager.
>
>This footnote also confirms that this email message has been swept by
>MIMEsweeper for the presence of computer viruses.
>
>www.mimesweeper.com
>**********************************************************************
>
>---





More information about the Methods mailing list