Antibody reduction?

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Thu Jan 31 20:22:57 EST 2002


On Thu, 31 Jan 2002, Ted M. wrote:

> It seems to me that COMPLETE reduction of the Ab should give only 50kDa
> (heavy chain) and 25kDa (light chain) bands but I haven't found
> conditions to do this yet. Is it possible?
>
I've run thousands of IPs and have never seen non-reduced antibody on my
gels. I use SDS load buffer with 250 mM DTT and heat the samples to ~70C

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

   Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
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