Antibody reduction?

[Kyle Legate]

On Thu, 31 Jan 2002, Ted M. wrote:

> It seems to me that COMPLETE reduction of the Ab should give only 50kDa
> (heavy chain) and 25kDa (light chain) bands but I haven't found
> conditions to do this yet. Is it possible?
>
I've run thousands of IPs and have never seen non-reduced antibody on my
gels. I use SDS load buffer with 250 mM DTT and heat the samples to ~70C

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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