extremely low copy number plasmid

[Kyle Legate]

On Thu, 31 Jan 2002, Trond Erik Vee Aune wrote:

> > I would appreciate any suggestions. I am working with a plasmid that is
> > in extremely low copy number. Lately we have been trying to increase the
> > yeild of our preps. Normal mini-preps give us nothing - no visible
>
You can amplify with chloramphenicol, like the previous poster suggested,
or you can amplify with spectinomycin (50ug/ml) for several hours before
harvesting the bugs. Both drugs serve to maximize production of your
plasmid.

... . . .  .  .  .    .    .    .     .     .     .      .      .      .
legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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