question about T vector cloning of PCR products

guozhiru1 at yahoo.com guozhiru1 at yahoo.com
Fri Jul 5 02:48:59 EST 2002


Dear All,
I cloned a PCR product into a commercialized T
vector(pUCm-T, from Sangon), but after sequencing, I
found that on one end of the insert, both the 3'A
overhang of the insert and the conresponding T
overhang on the T vector were lost. At first, I wonder
there is a sequencing mistake, but 2 independent
sequencing give the same result. 
I would like to know, have anyone encountered the same
problem? what is the mechanism?

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