DNA extraction from agarose

Bertrand Collet b.collet at abdn.ac.uk
Fri Jul 5 08:53:38 EST 2002


Hi Rene,

We use routinely  the combination agarose/TBE and Qiagen gel extraction
kit and it gives >90 % recovery,
I heard that the type of running buffer is important, maybe worth trying
to run in TBE,

Bertrand

Rene wrote:

> Hi lads,
>
> Probably not the first time somebody comes up with this, does anybody
> know a good way to get DNA out of agarose without too much trouble??
>
> I tried Qiagen's gel extraction kit with agarose/TAE, and I'm
> disapointed with the yield. Can the buffer go off or so?
>
> I've read of a method somewhere where you keep the gel slice in liquid
> nitrogen and then spin down. The supernatant could then be EtOH
> precipetated. Is the purity then ok?? What about cleaning the
> supernatant over a PCR puri column, anybody had a try with that??
>
> Thanks, Rene

--
Dr Bertrand Collet
Fish immunology group
Zoology Department
Tillydrone avenue
Aberdeen AB24 2TZ
Scotland - UK

Tel (office): (+44)(0)(1224)27 3796
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