Yeast's RNA extraction

sabina at qb.fcen.uba.ar sabina at qb.fcen.uba.ar
Fri Jul 5 13:42:03 EST 2002


Hi,
I am trying to extract RNA from yeast. In the extraction buffer I intend to use aurintricarboxylic acid (ATA) as a RNase inhibitor. The problem is that when I measure the absorbance at 260 and 280 nm it seems to interfere. Can anyone give me a method to "clean up" the RNA before I quantitate it?

Thank you very much
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