RNA extraction from pancreas

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Mon Jul 8 18:38:29 EST 2002


On Mon, 08 Jul 2002 23:15:28 +0200, Roland Hubner
<roland.hubner at ua.ac.be> wrote:

>Hi Daniel,
>
> RNA extraction from pancreas can be tremendously difficult depending on 
>species (activity/quantity of RNase; plenty in acini) but you are not in 
>this case  ;^)
>
> 10 years back (TRIzol just appeared on market) I had no troubles to 
>isolate it from rat pancreas with acidic phenol according to the now 
>classic acidic phenol method of Piotr Chomczynski... one step, 
>monophasic solution [just fill 50 ml tube with GITC, some salt, add the 
>water & phenol] or two step worked fine...

In fact, in the original GITC paper (Chirgwin et al) the GITC
homogenization was invented in order to isolate RNA from pancreas.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu



More information about the Methods mailing list