Fungus RNA isolation

[Volker Jacobi]

Greetings,

we are using Trizol Reagent for isolation of total RNA from a fungus that 
can be either grown as yeast or as mycelium. RNA extraction from yeast 
cells works well. But when we work with mycelium we get a lower yield and 
multiple bands (degradation?). We follow the standard Trizol protocol 
provided with the reagent. Our tissue is prepared as follows: grown on 
cellophane over solid medium, harvested, ground to powder in liquid 
nitrogen and stored at -80C.

Do you have any suggestions/tricks on how to isolate RNA from mycelium 
using Trizol that could improve quantity and quality of the RNA?

Best wishes,
Volker


Volker Jacobi, PhD
Université Laval
Récherche en sciences de la vie et de la santé
Centre de recherche en biologie forèstière
2212 Pavillon C.-E. Marchand
Sainte-Foy, Québec, Canada G1K 7P4

Téléphone: 418.656.2131 (postes 12521, 4598, 4403)
Télécopieur: 418.656.7493

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