DNA extraction from agarose

Iva Toudjarska toudjarska at wi.mit.edu
Thu Jul 11 09:08:35 EST 2002


Hi Rene,

Qiagen really gives low yield especially with long DNA fragments. I found
that glass milk techniques  work constantly well. And the good thing is
there is kit form Bio101- Geneclean Spin Kit- www.qbiogene.com even if
you don't have a lot of initial material you'll get everything back.

good luck
Iva


Rene wrote:

> Hi lads,
>
> Probably not the first time somebody comes up with this, does anybody
> know a good way to get DNA out of agarose without too much trouble??
>
> I tried Qiagen's gel extraction kit with agarose/TAE, and I'm
> disapointed with the yield. Can the buffer go off or so?
>
> I've read of a method somewhere where you keep the gel slice in liquid
> nitrogen and then spin down. The supernatant could then be EtOH
> precipetated. Is the purity then ok?? What about cleaning the
> supernatant over a PCR puri column, anybody had a try with that??
>
> Thanks, Rene




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