Glycerol stock preparation

Duncan Clark junk at hgmp.mrc.ac.uk
Sun Jul 14 14:46:41 EST 2002


Historians believe that in newspost 
<20020714084438.5591.qmail at ww02.hostica.com> on Sun, 14 Jul 2002, 
a_ishrath at yahoo.com penned the following literary masterpiece:
>i want to know the percentage of glycerol used to store E.col cultures and the ratio of glycerol is to the culture.

We use 2 volumes media to 1 volume 50% (v/v) glycerol. Has worked for 20 
odd years so far on my earliest stocks :-))

Duncan

-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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