DNA extraction from agarose

Mr JN Goulding jgouldin at hgmp.mrc.ac.uk
Mon Jul 15 06:48:30 EST 2002


We also regularly use the qiagen extraction columns in TAE gels
with very good yield

On Fri, 5 Jul 2002, Bertrand Collet wrote:

> Hi Rene,
>
> We use routinely  the combination agarose/TBE and Qiagen gel extraction
> kit and it gives >90 % recovery,
> I heard that the type of running buffer is important, maybe worth trying
> to run in TBE,
>
> Bertrand
>
> Rene wrote:
>
> > Hi lads,
> >
> > Probably not the first time somebody comes up with this, does anybody
> > know a good way to get DNA out of agarose without too much trouble??
> >
> > I tried Qiagen's gel extraction kit with agarose/TAE, and I'm
> > disapointed with the yield. Can the buffer go off or so?
> >
> > I've read of a method somewhere where you keep the gel slice in liquid
> > nitrogen and then spin down. The supernatant could then be EtOH
> > precipetated. Is the purity then ok?? What about cleaning the
> > supernatant over a PCR puri column, anybody had a try with that??
> >
> > Thanks, Rene
>
> --
> Dr Bertrand Collet
> Fish immunology group
> Zoology Department
> Tillydrone avenue
> Aberdeen AB24 2TZ
> Scotland - UK
>
> Tel (office): (+44)(0)(1224)27 3796
> Tel (lab): (+44)(0)(1224)27 2870
> Fax: (+44)(0)(1224)27 2396
>
>
>




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