C-terminal fusion vector

Frank O. Fackelmayer Frank at Fackelmayer.de
Mon Jul 15 10:08:54 EST 2002


Hi all,
does anyone out there know of and/or use a prokaryotic expression vector
that allows cloning of a fusion construct with the fusion partner at the
_carboxy_ terminus and the protein of interest at the amino terminus ?
The fusion partner should be greater than 12kD in size.
It seems there are only vectors available that are the other way round. Strange...

Frank



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