C-terminal fusion vector

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Mon Jul 15 10:42:26 EST 2002


Novagen (no affiliation) does have a pET vector, pET38, that has a 
C-terminal fusion to cellulose binding protein.  The tag is supposed 
to be approximately 107 amino acids, close but a bit shy of the 12 
kDa minimum you're looking for.


>Hi all,
>does anyone out there know of and/or use a prokaryotic expression vector
>that allows cloning of a fusion construct with the fusion partner at the
>_carboxy_ terminus and the protein of interest at the amino terminus ?
>The fusion partner should be greater than 12kD in size.
>It seems there are only vectors available that are the other way 
>round. Strange...


Michael L. Sullivan, 
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax

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