C-terminal fusion vector
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Mon Jul 15 10:42:26 EST 2002
Novagen (no affiliation) does have a pET vector, pET38, that has a
C-terminal fusion to cellulose binding protein. The tag is supposed
to be approximately 107 amino acids, close but a bit shy of the 12
kDa minimum you're looking for.
>does anyone out there know of and/or use a prokaryotic expression vector
>that allows cloning of a fusion construct with the fusion partner at the
>_carboxy_ terminus and the protein of interest at the amino terminus ?
>The fusion partner should be greater than 12kD in size.
>It seems there are only vectors available that are the other way
Michael L. Sullivan,
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5144 Phone
(608) 264-5147 Fax
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