Growing HeLa Cells in a spinner

Jes Kuruvilla jkuruvil at
Mon Jul 15 15:34:25 EST 2002

Hi all,
Does anybody know how to get He La cells (spinner adapted)
to grow successfully? I need to make some nuclear extract. I
have tried ( Ca- deficient) Jokliks media with 10% fetal
Calf serum and have been keeping the density at 2X 10^5.
They still seem to be just sitting around in the flask and
not doubling. Most of the cells are found in clumps. Is this
usual for cells taken from a trypsinized 10 cm plate??

Thanks in advance for all your replies!


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