Gene Quantification in Endocrinology 18. July 2002

genequantification gene.quantification at wzw.tum.de
Thu Jul 18 05:49:23 EST 2002


Gene Quantification in Endocrinology   18. July 2002 

NEW features on    http://www.wzw.tum.de/gene-quantification/

Endocrinology  -  NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/endo.html
NEW ENDOCRINOLOGY POSTERS   on  Poster Boards
http://www.wzw.tum.de/gene-quantification/poster-a.html
Housekeeping Gene & Normalisation in kinetic PCR
http://www.wzw.tum.de/gene-quantification/hkg.html
NEW VERSIONS  of   REST©   and  REST-XL©
http://www.wzw.tum.de/gene-quantification/rest.html
Verification of array results  -  NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/array.html
real-time  PCR  and  RT-PCR  -  NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/real-time.html
PCR, RPA, Northern-Blot & Southern-Blot   MOVIES
http://www.wzw.tum.de/gene-quantification/movie.html

and external links:
Q-Gene Software    Processing of gene expression data generated by
quantitative real-time RT-PCR
TATAA Biocenter:  q-PCR  Network of Excellence
NEW real-time PCR primer and probe Database


Main focus of GENE QUANTIFICATION web page is to describe
and summarize all technical aspects involved in quantitative 
gene expression analysis using real-time PCR  &  RT-PCR.
( specificity, sensitivity, reproducibility, intra- & inter-assay
variations,
kinetic PCR efficiency calculation, optimisation strategy, etc.)

The GENE QUANTIFICATION web page illustrates the usefulness of a
reliable
quantification strategy, and the difference between absolute vs.
relative quantification
assays in kinetic PCR & kinetic RT-PCR.

RT-PCR is the technique of choice for analysing mRNA in extremely low
abundance.
Real-time RT-PCR using SYBR Green I detection combines the ease and
necessary
exactness to be able to produce reliable as well as rapid results. 
To obtain high accuracy and reliability in RT and real-time PCR highly
defined
quantification strategies and calculation methods are needed.



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