Questions from a beginner
dknorr at lynxgen.com
Mon Jul 22 10:58:10 EST 2002
"Philipp Wechner" <philipp.wechner at uibk.ac.at> wrote in message
news:3D3BB674.F77B1DBA at uibk.ac.at...
> > SDS,
> denaturates proteins to get them out of your sample
Proteins like, say RNases
Same as a pH buffer? Never seen this spelling.
> > betamercaptoethanol,
> not sure what this does in an RNA-extraction...
Historically, it is added to reduce the cysteines in RNases, rendering them
> > DEPC,
> to get rid of RNase
Diethyl pyrocarbonate at about 0.1% inactivates RNase and many other
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