Questions from a beginner

Dave Knorr dknorr at
Mon Jul 22 10:58:10 EST 2002

"Philipp Wechner" <philipp.wechner at> wrote in message
news:3D3BB674.F77B1DBA at
> > SDS,
> denaturates proteins to get them out of your sample
Proteins like, say RNases

> puffer
Same as a pH buffer?  Never seen this spelling.

> > betamercaptoethanol,
> not sure what this does in an RNA-extraction...
Historically, it is added to reduce the cysteines in RNases, rendering them

> > DEPC,
> to get rid of RNase
Diethyl pyrocarbonate at about 0.1% inactivates RNase and many other

Dave Knorr

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