Questions from a beginner

Dave Knorr dknorr at lynxgen.com
Mon Jul 22 10:58:10 EST 2002


"Philipp Wechner" <philipp.wechner at uibk.ac.at> wrote in message
news:3D3BB674.F77B1DBA at uibk.ac.at...
> > SDS,
> denaturates proteins to get them out of your sample
Proteins like, say RNases

> puffer
Same as a pH buffer?  Never seen this spelling.

> > betamercaptoethanol,
> not sure what this does in an RNA-extraction...
Historically, it is added to reduce the cysteines in RNases, rendering them
inactive.

> > DEPC,
>
> to get rid of RNase
Diethyl pyrocarbonate at about 0.1% inactivates RNase and many other
proteins.

Dave Knorr





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