Questions from a beginner

Philipp Wechner philipp.wechner at uibk.ac.at
Tue Jul 23 02:45:53 EST 2002



Dave Knorr schrieb:

> "Philipp Wechner" <philipp.wechner at uibk.ac.at> wrote in message
> news:3D3BB674.F77B1DBA at uibk.ac.at...
> > > SDS,
> > denaturates proteins to get them out of your sample
> Proteins like, say RNases
>
> > puffer
> Same as a pH buffer?  Never seen this spelling.

i have seen this spelling - in german :) i allways get messed up with the
spellings:
german = puffer
english = buffer

>
>
> > > betamercaptoethanol,
> > not sure what this does in an RNA-extraction...
> Historically, it is added to reduce the cysteines in RNases, rendering them
> inactive.
>
> > > DEPC,
> >
> > to get rid of RNase
> Diethyl pyrocarbonate at about 0.1% inactivates RNase and many other
> proteins.
>
> Dave Knorr




More information about the Methods mailing list