Questions from a beginner
philipp.wechner at uibk.ac.at
Tue Jul 23 02:45:53 EST 2002
Dave Knorr schrieb:
> "Philipp Wechner" <philipp.wechner at uibk.ac.at> wrote in message
> news:3D3BB674.F77B1DBA at uibk.ac.at...
> > > SDS,
> > denaturates proteins to get them out of your sample
> Proteins like, say RNases
> > puffer
> Same as a pH buffer? Never seen this spelling.
i have seen this spelling - in german :) i allways get messed up with the
german = puffer
english = buffer
> > > betamercaptoethanol,
> > not sure what this does in an RNA-extraction...
> Historically, it is added to reduce the cysteines in RNases, rendering them
> > > DEPC,
> > to get rid of RNase
> Diethyl pyrocarbonate at about 0.1% inactivates RNase and many other
> Dave Knorr
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