guanidine and isopropanol freeze solid - why?

jon at jon at
Tue Jul 23 07:34:41 EST 2002

Precipitate in an ice bath then....besides, GCN comes out of solution at low temperatures...esp. if its in a saturated solution, which in your case it probably is in the (aq) phase...

Jacob Hofma-Bang wrote:

> Hi.

> Doing RNA extraction with guanidine thiscyanate and phenol, I precipit with  
> isopropanol. However it freezes solid at -20 C.

> Any suggestions?

> Jacob Hofman-Bang, Ph.D.

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