ballal_sv at hotmail.com
Sat Jul 27 21:51:20 EST 2002
Please excuse me if you find my question basic. I am not an expert in
molecular biology but can RNAse survive a good autoclavation cycle???
I know it is heat resistant but after 20- 30 min or say 60 min at 121
C will it be still active ?
mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote in message news:<email@example.com>...
> I've found that water processed with a well-maintained MilliQ system
> is RNAse free without any further treatment. If you don't have a
> comparable water treatment system, you could perhaps find a collegue
> who does and get your "RNase-free" water from him or her.
> >we regularly treat deionized water with 0.01% DEPC to render it
> >RNase-free. Yet, we are also aware that DEPC residues can modify
> >nucleic acids. So we are looking for alternatives to DEPC-treatment.
> >Is passing water through a 0.2-? filter disk a safe way to eliminate RNas
> Michael L. Sullivan,
> U.S. Dairy Forage Research Center
> 1925 Linden Drive West
> Madison WI, 53706
> (608) 264-5144 Phone
> (608) 264-5147 Fax
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