RNase-free water

S.Ballal ballal_sv at hotmail.com
Sat Jul 27 21:51:20 EST 2002


Please excuse me if you find my question basic.  I am not an expert in
molecular biology but can RNAse survive a good autoclavation cycle???

I know it is heat resistant but after 20- 30 min or say 60 min at 121
C will it be still active ?


S.Ballal
Ahmedabad, India


mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote in message news:<p05111a00b9675424e685@[144.92.64.249]>...
> I've found that water processed with a well-maintained MilliQ system 
> is RNAse free without any further treatment.  If you don't have a 
> comparable water treatment system, you could perhaps find a collegue 
> who does and get your "RNase-free" water from him or her.
> 
> Mike
> 
> 
> >Hi,
> >
> >we regularly treat deionized water with 0.01% DEPC to render it 
> >RNase-free. Yet, we are also aware that DEPC residues can modify 
> >nucleic acids. So we are looking for alternatives to DEPC-treatment.
> >
> >Is passing water through a 0.2-? filter disk a safe way to eliminate RNas
>  es?
> >
> >Greetings,
> >Volker
> >
> >---
> 
> 
> -- 
> 
> Michael L. Sullivan, 
> Ph.D                                                            
> U.S. Dairy Forage Research Center
> 1925 Linden Drive West
> Madison WI, 53706
> 
> (608) 264-5144 Phone
> (608) 264-5147 Fax
> ---



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