In vitro transcription buffer

CM q6252397 at fernuni-hagen.de
Sun Jul 28 12:55:25 EST 2002


Dear all,

does anybody have a receipe for an in vitro transcription buffer (especially
for E. coli RNA polymerase)??

I also have no idea how to prepare such a buffer without RNase
contamination.
Do you have any hints for that problem?

TIA,
Tina





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