T vector clone sequencing problem help!!!

guangming ye gmye at yahoo.com
Mon Jul 29 08:55:45 EST 2002


I was used pMD18-T Vector (from TaKaRa,Japan) clone to
sequence a 1Kb gene .The PCR product added T/A ,
cloned into T vector and transformed DH5-alpha
completed cell.Sequence result shows the DNA sequence
is not my sequence ,but size and including prime
sequence are the same with mine.who can tell me any
tip about this .Thank for any help!!

Bright Yeh 

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