RNase-free water

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Mon Jul 29 09:46:10 EST 2002


Quoting Michael Witty <mw132 at mole.bio.cam.ac.uk>:

> On Mon, 29 Jul 2002, Duncan Clark wrote:
> 
> > >we regularly treat deionized water with 0.01% DEPC to render it
> > >RNase-free.
> >
> > Presumably you are autoclaving it which if so does not leave anything
> > other than ethanol behind. If you do not autoclave then that level of
> > DEPC could certainly cause problems.
> >
> > Duncan
> 

Duncan, do you believe that etoh will be "left behind" aftr autoclavng?  


-- 
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
---



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