T vector clone sequencing problem help!!!

[zhong.huang at mail.tju.edu]

guangming ye wrote:

> Hello,

> I was used pMD18-T Vector (from TaKaRa,Japan) clone to
> sequence a 1Kb gene .The PCR product added T/A ,
> cloned into T vector and transformed DH5-alpha
> completed cell.Sequence result shows the DNA sequence
> is not my sequence ,but size and including prime
> sequence are the same with mine.who can tell me any
> tip about this .Thank for any help!!

> Bright Yeh 

Your PCR gives you non-specific products. You may try to optimize your PCR reaction or design new primer pairs.

Zhong



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