RNase-free water

Emir Khatipov khatipovNO at NOuchicago.edu
Mon Jul 29 15:12:02 EST 2002


Read the link I posted in my first message in this thread. It answers all
the questions that had been risen in this thread so far.
Emir

"Roland Hübner" <roland.hubner at ua.ac.be> wrote in message
news:3d459da6 at news.uia.ac.be...
> > > Presumably you are autoclaving it which if so does not leave anything
> > > other than ethanol behind. If you do not autoclave then that level of
> > > DEPC could certainly cause problems.
> &
> > I agree that using DEPC without autoclaving is dangerous, experiment
wise.
> > Even with autoclaving I could only do RNA work with the water.  In my
more
> > naive days, I figured that DEPC autoclaved water if it was good for RNA
then
> > it must be better for DNA -- *wrong*...  I had to repeat everything that
day
> > using autoclaved water.
>
>  Whoaw! but how long did you autoclave DEPC-spiked water?
>
>  On a sideline, does somebody know what the fruity smell of autoclaved
> DEPC-water comes from?
>
> Greetings,
>  Roland





More information about the Methods mailing list