khatipovNO at NOuchicago.edu
Mon Jul 29 15:12:02 EST 2002
Read the link I posted in my first message in this thread. It answers all
the questions that had been risen in this thread so far.
"Roland Hübner" <roland.hubner at ua.ac.be> wrote in message
news:3d459da6 at news.uia.ac.be...
> > > Presumably you are autoclaving it which if so does not leave anything
> > > other than ethanol behind. If you do not autoclave then that level of
> > > DEPC could certainly cause problems.
> > I agree that using DEPC without autoclaving is dangerous, experiment
> > Even with autoclaving I could only do RNA work with the water. In my
> > naive days, I figured that DEPC autoclaved water if it was good for RNA
> > it must be better for DNA -- *wrong*... I had to repeat everything that
> > using autoclaved water.
> Whoaw! but how long did you autoclave DEPC-spiked water?
> On a sideline, does somebody know what the fruity smell of autoclaved
> DEPC-water comes from?
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