junk at hgmp.mrc.ac.uk
Tue Jul 30 05:24:25 EST 2002
Historians believe that in newspost
<1027954167.3d4555f7f203e at webmail.nmsu.edu> on Mon, 29 Jul 2002, Dr.
Hiranya S. Roychowdhury <hroychow at nmsu.edu> penned the following
>Quoting Michael Witty <mw132 at mole.bio.cam.ac.uk>:
>> On Mon, 29 Jul 2002, Duncan Clark wrote:
>> > >we regularly treat deionized water with 0.01% DEPC to render it
>> > >RNase-free.
>> > Presumably you are autoclaving it which if so does not leave anything
>> > other than ethanol behind. If you do not autoclave then that level of
>> > DEPC could certainly cause problems.
>> > Duncan
>Duncan, do you believe that etoh will be "left behind" aftr autoclavng?
Note to self, never make statements.
Having said that what does happen to Ethanol/ethanol vapour when
autoclaved. I can't remember my chemistry from many many years ago.
For more info. on the vagaries of DEPC check out:
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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