28S doesn't blot!

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Jun 5 18:01:21 EST 2002


On Wed, 05 Jun 2002 19:04:46 GMT, "Stefi~" <gifalone at tin.it> wrote:

>I use a 50mL gel with 2,5ug EtBr (approx height: 6mm) and my RNA is from
>Bufo bufo. I B.bufo's 28S doesn't have a nick because I've been making RNA
>denaturing gel electrophoresis analysis with that species for almost 2 years
>and 2 clear bands have always appeared.
>I'll try less EtBr and a thiner gel.
>Thanx a lot for all your suggests.

A I kg weight for a 50 ml gel sounds way too heavy. I'd bet you are
squashing the gel flat within minutes of transfer. Use a much lighter
weight. 

Nick


-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu




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