express tac promoter in BL21(DE3)

Duncan Clark junk at hgmp.mrc.ac.uk
Thu Jun 6 09:23:04 EST 2002


Historians believe that in newspost 
<j9oufuknkde1ab06hra5k0fdsqk38lhupl at 4ax.com> on Thu, 6 Jun 2002, 
gianluca <gianluca.molla-rimuovere- at uninsubria.it> penned the following 
literary masterpiece:
>Since I want to replicate this experiment, do you think that there
>could be problems with T7 RNA pol expression after IPTG addition?

Probably not as it doesn't get produced that much i.e. I haven't really 
noticed a T7 RNA pol band on SDS PAGE.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.




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