express tac promoter in BL21(DE3)

Thu Jun 6 10:10:52 EST 2002

gianluca wrote:

> Hi, I read in a paper that a vector with Ptac promoter (pMal) was
> expressed in BL21(DE3) cells.
> Since I want to replicate this experiment, do you think that there
> could be problems with T7 RNA pol expression after IPTG addition?

I don't know, but IMHO  it shouldn't.

> In case of affirmative answer, can you suggest a E. coli strain I
> could use? I thought of JM109.

I've used some different strains to protein overexpression. UT5600 (OmpT 
mutant)works just fine with the pMAL system (actually, it's the suggested one by 


More information about the Methods mailing list