immunoprecipitation

shen wang ben2162002 at yahoo.com
Fri Jun 7 18:36:21 EST 2002


Hi

I immunoprecipitated my protein with rabbit antibody
and the upperpart of the membrane I probed with rabbit
antibody and lower part with mouse antibody. I saw the
heavy chain of IgG in the upper part but no light
chain in the lower part of the membrane. What could be
the reason I did not see any IgG small chain of 25
kDa.

ben

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