Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Mon Jun 10 08:18:53 EST 2002
As others have pointed out, this sounds like using too much EtBr in
the gel. I usually use 1 ul of a 10 mg/ml stock EtBr per 100 ml of
gel. In my hands, this gives good staining without excessive
background. You may also note that sometimes old stock solutions can
become more concentrated and/or have EtBr crystals form in them, in
which case it's easy to end up with too much EtBr in the gel.
Hope this helps.
>Some people in the lab I work have some problems with the quality of
>their EtBr-Agarose gel. The top part seems completely saturated with
>EtBr and give a glow making image analysis very difficult.
>Is there too much EtBr ? Or another explanation ?
>It looks like it is very common problem but I never had an explanation
>Thanks in advance for your time,
More information about the Methods