transformation problem

satya lakshmi satyashreee at
Wed Jun 12 02:41:26 EST 2002

iam facing problem with ligation of a cloned vector and
insert both are PshA1/Bstap1 digested. but when i transfrom my ligated
product i am not getting any colonies while the ligated product shows
a band above the vector running like a relaxed circular form when
compared with the undigested.
can anyone help me as aim unable to understand what could be the
possible problem?

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