SerREMIVETHISAln at netscape.net
Wed Jun 12 10:35:36 EST 2002
Are you sure that band is a oc form?.
A possible explanation would be that one of the enzymes is not cutting properly
on the vector or on the insert, and what you get is the ligation product of two
vectors, two inserts or vector+insert (linearized). As you are not getting any
religated vector I would say that the vector restriction sites are OK. Check the
sites on the insert.
If everything seems to be OK, I would start to think on toxicity problems of the
satya lakshmi wrote:
> iam facing problem with ligation of a cloned product.my vector and
> insert both are PshA1/Bstap1 digested. but when i transfrom my ligated
> product i am not getting any colonies while the ligated product shows
> a band above the vector running like a relaxed circular form when
> compared with the undigested.
> can anyone help me as aim unable to understand what could be the
> possible problem?
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