problem with minipreps

David L Haviland David.L.Haviland at uth.tmc.edu
Wed Jun 19 09:57:27 EST 2002


Tatsuya:

An EXCELLENT point that I overlooked.   I've done so many that it is
instinct not to overload, but that is a problem with beginning people.  If
the protocol says 5-10 mls, many will try to squeeze 20...  I've had the
best results using the low end of what is recommended.

David

----- Original Message -----
From: ""MAEDA, Tatsuya"" <maeda at ims.u-tokyo.ac.jp>
To: <methods at hgmp.mrc.ac.uk>
Sent: Tuesday, June 18, 2002 20:42
Subject: Re: problem with minipreps


> My bet is that they tried to treat too much bacteria for the regular-scale
> mini-preps.  Out of capacity.  It sometimes happened when I used super
rich
> media (TB).
>
> TM
>
> ---
>

---




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