A comparison between AgeI and BshTI

Duncan Clark junk at hgmp.mrc.ac.uk
Thu Jun 20 04:28:39 EST 2002

Historians believe that in newspost 
<3D117AC5.8000502 at chembio.REMOVETHISBEFOREREPLYING.ntnu.no> on Thu, 20 
Jun 2002, Trond Erik Vee Aune 
<trondaun at chembio.REMOVETHISBEFOREREPLYING.ntnu.no> penned the following 
literary masterpiece:
>There should be a database for enzymes which shows the activity of 
>different isoscizomers compared.

The problem is that there are tens of isoschizomers out there, the vast 
majority un-commercialised. I must have at least a  hundred from our 
screenings, some of which have been licensed off, the rest just sit 
there and have never been made in more than mostly crude extracts for 
screening. There are 586 RE's commercially available. If you have the 
time and money the iso's could be compared but ......

I don't know if REBASE (http://rebase.neb.com/rebase/) has more info. on 
iso. comparisons but I would doubt it.

REBASE enzyme #: 3829
Prototype: AgeI
Org #: 592
Organism source: V. Butkus
Organism: Bacillus sphaericus Jo22-024
Growth Temperature: 37 °
Exhibits star activity

BshT I is available from Fermentas, but note the star activity :-((

More statistics courtesy of REBASE

Type II   Restriction Enzymes           3499
Prototype Type II  REs          238
Cloned Type II REs                      346

I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

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