A comparison between AgeI and BshTI
junk at hgmp.mrc.ac.uk
Thu Jun 20 04:28:39 EST 2002
Historians believe that in newspost
<3D117AC5.8000502 at chembio.REMOVETHISBEFOREREPLYING.ntnu.no> on Thu, 20
Jun 2002, Trond Erik Vee Aune
<trondaun at chembio.REMOVETHISBEFOREREPLYING.ntnu.no> penned the following
>There should be a database for enzymes which shows the activity of
>different isoscizomers compared.
The problem is that there are tens of isoschizomers out there, the vast
majority un-commercialised. I must have at least a hundred from our
screenings, some of which have been licensed off, the rest just sit
there and have never been made in more than mostly crude extracts for
screening. There are 586 RE's commercially available. If you have the
time and money the iso's could be compared but ......
I don't know if REBASE (http://rebase.neb.com/rebase/) has more info. on
iso. comparisons but I would doubt it.
REBASE enzyme #: 3829
Org #: 592
Organism source: V. Butkus
Organism: Bacillus sphaericus Jo22-024
Growth Temperature: 37 °
Exhibits star activity
BshT I is available from Fermentas, but note the star activity :-((
More statistics courtesy of REBASE
Type II Restriction Enzymes 3499
Prototype Type II REs 238
Cloned Type II REs 346
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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