problem with minipreps
khatipovNO at NOuchicago.edu
Mon Jun 24 17:50:26 EST 2002
I bet they overload the preps. As already suggested, use less cells per
prep. Otherwise, you might want to use spin filters similar to ones used in
some glassmilk kits to separate the insoluble precipitate from the liquid
plasmid fraction. These filters look the same as miniprep columns, but have
non-binding filter instead of binding resin. Check Geneclean kits from
Bio101 for example (no affil.).
"Carol Chihara" <chihara at usfca.edu> wrote in message
> My students are having trouble with alkaline lysis minipreps. They
> get an extremely viscous DNA solution after adding the KAc (P3). i.e.
> the mass of membranes and genomic DNA is not pelleting. Any
> suggestions as to what the problem might be?
> Carol Chihara, Professor
> Department of Biology
> University of San Francisco
> 2130 Fulton St.
> San Francisco, CA 94117-1080
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