Will I get amplification if my last 3' nucleotide is not paired?

Philipp Wechner philipp.wechner at uibk.ac.at
Thu Jun 27 10:41:33 EST 2002



Sylvain Foisy schrieb:

> Hi,
>
> I am trying to isolate clones from transfection experiments with PCR and
> I have lots of background with a faint band that is at the right
> molecular weight. Is it possible to get amplification if the last
> nucleotide of one of my primers (at the 3' end) does not hybridize with
> the template?

dont think that this works....

>
>
> Any insight would help
>
> Sylvain Foisy
> U. de Montreal




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