Home made quantitatice real time RT-PCR? SYBR Green?

Duncan Clark junk at hgmp.mrc.ac.uk
Fri Jun 28 04:48:04 EST 2002

Historians believe that in newspost 
<20020627221947.11893.qmail at ww02.hostica.com> on Thu, 27 Jun 2002, 
idelgado at bcm.tmc.edu penned the following literary masterpiece:
>My question is: does anybody out there have experience with the amount of Sybr green that needs to be used per reaction?. We would like to set up
>our own reactions and the sole component that we have no idea about how to use is Sybr Green. Any help in this regard is greatly appreciated.

40,000 to 66,000 fold dilution (final in PCR) of the stock Sybr Green I 
from Mol probes.

Pay a visit to the qpcrlistserver on www.yahoogroups.com

I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

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