Isoform vs pseudogene

[Joseph C. Bagshaw]

If the gene in question is transcribed to produce an mRNA it clearly is
not a processed pseudogene.  A processed pseudogene has not promoter, thus
can not be transcribed, thus alternative splicing is a moot point.  If the
gene is a pseudo that resulted from sequence divergence, you will have to
clone and sequence the suspect mRNA.

********************  HAVE GENES, WILL TRAVEL  ********************
Joe Bagshaw, Worcester Polytechnic Institute
jbagshaw at wpi.edu
Roadkill on the information superhighway.

On Mon, 25 Feb 2002, Martin Chan wrote:

> Dear netters,
>     How to experimentally prove an alternatively spliced mRNA isn't a
> pseudogene?
>
> Thanks in advance.
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