AFLP-DNA extraction
Lorena Quintero
lquinterob at hotmail.com
Fri Mar 1 11:15:59 EST 2002
Hello all,
We are doing a study with AFLP in Caryodendron genus, it is an
amazonian species. We have assayed various DNA buffer extraction like
Afanador et. al., Dellaporta et al, Porebski et al, Bollard et al.,
Hillis et al. and Biorad without successful, this plant contains a
high quantity of phenol and other substances, we think that this
doesn't allow us to obtain a good quality of DNA or that it can digest
with restriction enzymes
Could anyone suggest me a protocol for DNA extraction?
I would appreciate.
Thank you
Lorena
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