Storing dNTPs at -70 or -20?

Duncan Clark junk at hgmp.mrc.ac.uk
Mon Mar 4 04:28:40 EST 2002


Historians believe that in newspost <3C8117B9.D03863C0 at mts.net> on Sat, 
2 Mar 2002, Andre Hamel <ahamel at mts.net> penned the following literary 
masterpiece:
>ThanX ... I will post any email responses if they are not posted
>already.

You should always store dNTPs as liquid not solid. In solid form, 
according to very old Pharmacia catalogues, they undergo a 
disproportionation reaction which means you get increasing levels of 
dNDP and dNMP at a surprisingly high level (1-2% per week).

I have always stored dNTPs stocks, by the hundreds of mls at 100mM, at
-20C (frost free!) and never had a problem in years.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.




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