Storing dNTPs at -70 or -20?

Nicolas Kuperwasser nkuperw at yahoo.com
Mon Mar 4 10:26:20 EST 2002


Duncan Clark <junk@[127.0.0.1]> wrote in message news:<m8JiSK5I5zg8EAvV@[127.0.0.1]>...
> Historians believe that in newspost <3C8117B9.D03863C0 at mts.net> on Sat, 
> 2 Mar 2002, Andre Hamel <ahamel at mts.net> penned the following literary 
> masterpiece:
> >ThanX ... I will post any email responses if they are not posted
> >already.
> 
> You should always store dNTPs as liquid not solid. In solid form, 
> according to very old Pharmacia catalogues, they undergo a 
> disproportionation reaction which means you get increasing levels of 
> dNDP and dNMP at a surprisingly high level (1-2% per week).
> 
> I have always stored dNTPs stocks, by the hundreds of mls at 100mM, at
> -20C (frost free!) and never had a problem in years.
> 
> Duncan

the only other thing to make sure of is to pH them to neutrality
before freezing, then they'll be stable for a long time.




More information about the Methods mailing list