Storing dNTPs at -70 or -20?
nkuperw at yahoo.com
Mon Mar 4 10:26:20 EST 2002
Duncan Clark <firstname.lastname@example.org> wrote in message news:<m8JiSK5I5zg8EAvV@[127.0.0.1]>...
> Historians believe that in newspost <3C8117B9.D03863C0 at mts.net> on Sat,
> 2 Mar 2002, Andre Hamel <ahamel at mts.net> penned the following literary
> >ThanX ... I will post any email responses if they are not posted
> You should always store dNTPs as liquid not solid. In solid form,
> according to very old Pharmacia catalogues, they undergo a
> disproportionation reaction which means you get increasing levels of
> dNDP and dNMP at a surprisingly high level (1-2% per week).
> I have always stored dNTPs stocks, by the hundreds of mls at 100mM, at
> -20C (frost free!) and never had a problem in years.
the only other thing to make sure of is to pH them to neutrality
before freezing, then they'll be stable for a long time.
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