Zinc with NTA resin ?

Peter Cherepanov peter.cherepanov at uz.kuleuven.ac.be
Tue Mar 5 04:18:56 EST 2002


People, has anyone used Qiagen's NTA resin for Zinc chelation ?

My reason tells me that since NTA has 4 groups to chelate a metal, Zinc will
have none left to bind a protein. On the other hand, may be Zn will actually
only complex 3 of them, because of the requirenment for a planar structure
of the complex and will still keep 1 valence left ...  (Zinc has a
coordination number 4)

.... I will probably have to order an IDA resin.

Peter

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