Retrovirus with LTR-polyA-LTR = garbage?

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Tue Mar 5 21:57:07 EST 2002




Nick wrote:
> Question...is the polyA a trac of polyA or the cis-element signalling for
> polyA'tion to occur?  My feeling is that a strech of A's will not affect the
> proper processing of the RNA and subsequent virus formation, but a cis-element
> will pretty much muck up virus construction.
> 

Dear Nick,

it's SV40 poly A SIGNAL. AATAAA. 2 times. ouch!

what about other polyA signals frequently used like from bGH bovine growth 
hormone?

Wo
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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.o.C.

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