DMSO stored samples-inhibition of Restriction Digests

Michelle Waycott michelle.waycott at jcu.edu.au
Tue Mar 5 23:18:28 EST 2002


Hello all,
we are working with blood samples that have been stored in salt
saturated blood DMSO. Following a standard Proteinase K DNA extraction
and GeneClean cleanup of the DNA we find that we cannot get restriction
digests working.
Has anyone any ideas about how to overcome this problem by perhaps
cleaning the blood /DMSO solution first?

cheers
Michelle

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