Retrovirus with LTR-polyA-LTR = garbage?
aiyar at ebv.mimnet.northwestern.edu
Wed Mar 6 00:24:36 EST 2002
On 6 Mar 2002 02:57:07 -0000,
"Wolfgang Schechinger" (wolfsc at ibms.sinica.edu.tw) wrote:
> it's SV40 poly A SIGNAL. AATAAA. 2 times. ouch!
> what about other polyA signals frequently used like from bGH bovine growth
Same problem. The bGH polyA signal is also strong.
Completion of retroviral reverse transcription requires
that polyadenylation occur only AFTER the U3 and R
sequences in the 3' LTR.
Reverse-transcription, integration and expression will
occur if your gene is inserted in the MCS with no polyA
signals between the end of your gene and 3' LTR.
Ashok Aiyar, Ph.D.
Assistant Professor email: a-aiyar at northwestern.edu
Department of Microbiology-Immunology office: (312) 503-2524
303 E. Chicago Avenue, WARD 4-123 lab: (312) 503-2542
Northwestern University, Chicago, IL 60611 fax: (312) 503-1339
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