Retrovirus with LTR-polyA-LTR = garbage?

Ashok Aiyar aiyar at ebv.mimnet.northwestern.edu
Wed Mar 6 00:24:36 EST 2002


On 6 Mar 2002 02:57:07 -0000,
    "Wolfgang Schechinger" (wolfsc at ibms.sinica.edu.tw) wrote:

> it's SV40 poly A SIGNAL. AATAAA. 2 times. ouch!
> 
> what about other polyA signals frequently used like from bGH bovine growth 
> hormone?

Same problem.  The bGH polyA signal is also strong.
Completion of retroviral reverse transcription requires
that polyadenylation occur only AFTER the U3 and R
sequences in the 3' LTR.

Reverse-transcription, integration and expression will
occur if your gene is inserted in the MCS with no polyA
signals between the end of your gene and 3' LTR.

Later,
Ashok
-- 
Ashok Aiyar, Ph.D.
Assistant Professor                    email: a-aiyar at northwestern.edu
Department of Microbiology-Immunology           office: (312) 503-2524
303 E. Chicago Avenue, WARD 4-123                  lab: (312) 503-2542
Northwestern University, Chicago, IL 60611         fax: (312) 503-1339




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