blurry bands on SDS-PAGE

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Mar 6 10:28:02 EST 2002


. . . I think they are caused by residues with pKa's near the pH of the
running buffer, like Histidine (pKa 6.0) and cysteine (pKa of
thiol/thiolate (pKa about 9).  What do you think?  Regards, Mike.

(could it be a matter of solubility and caused by Phe and so on?).




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