blurry bands on SDS-PAGE
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Wed Mar 6 11:04:56 EST 2002
<Pine.SGI.4.33.0203061519360.2867729-100000 at mole.bio.cam.ac.uk>,
Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> . . . I think they are caused by residues with pKa's near the pH of the
> running buffer, like Histidine (pKa 6.0) and cysteine (pKa of
> thiol/thiolate (pKa about 9). What do you think? Regards, Mike.
> (could it be a matter of solubility and caused by Phe and so on?).
First I've heard of it. Consider what the SDS is doing - it's swamping
your protein's charge, so unless you've got something veeeeeeeeery
strange . . .
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk New: http://www.rg-d.com/BioLOG/
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