Dead stab culture - what to do?

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Mar 6 13:29:47 EST 2002


Cornelius Krasel <krasel at wpxx02.toxi.uni-wuerzburg.de> wrote in message news:<bbd56a.6gv.ln at wpxx02.toxi.uni-wuerzburg.de>...
> I seem to have a dead stab culture (at least I didn't manage to get
> any colonies from it by taking out some agar and spreading it on a
> plate) from which I would like to recover the plasmid somehow.
>

Sometimes I've had better luck with old stabs by innoculating them in
liquid culture (with no selection) first, and then streaking on to amp
(or whatever) plates.

Did it look dried out?

Nick




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