blurry bands on SDS-PAGE
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Thu Mar 7 04:02:37 EST 2002
In article <hUrh8.6$s4.387 at news.uchicago.edu>,
"Emir" <ekhatipoREMOVE at midway.uchicago.edu> wrote:
> Usual troubleshooting tips for cases like that are check that 1) you don't
> have too much salt in your sample,
That's often a cause of non-pretty gels.
> 2) don't overload the well,
Hmm that shouldn't make them blurry. . .
> 3) pH of your
> sample is 6.8 as that of the stacking buffer... Could your problem be
I'm not convinced that's a problem - I know I've loaded samples which
have turned the loading buffer yellow, but the buffering capacity of
the gel itself meant they ran OK. *Good* sigmonster, have a cookie.
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk New: http://www.rg-d.com/BioLOG/
'For every expert, there is an equal and opposite expert' A C Clarke
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